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KMID : 0379119840120040164
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Korean Journal of Mycology
1984 Volume.12 No. 4 p.164 ~ p.169
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Characteristics of Fusion Products between Pleurotus ostreatus and Pleurotus florida Following Interspecific Protoplast Fusion
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박용환/Park, Yong Hwan
유영복/유창현/고승주/변명옥/Peberdy, John F./Yoo, Young Bok/You, Chang Hyun/Go, Seung Joo/Byun, Myung Ok
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Abstract
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Interspecific heterokaryons were obtained between auxotrophic mutants of Pieurotus ostreatus and Pleurotus florida by polyethylene glycol-Ca^(++) induced fusion of somatic protuplasts. The fusion products produced colonies of dense growing mycelium after 10-14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.
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